Browsing by Author "Hancock, John T."

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Nitric oxide-releasing nanomaterials: from basic research to potential biotechnological applications in agriculture
(John Wiley & Sons, 2022) Seabra, Amedea B.; Silveira, Neidiquele M.; Ribeiro, Rafael V.; Pieretti, Joana C.; Barroso, Juan B.; Corpas, Francisco J.; Palma, José M.; Hancock, John T.; Petřivalský, Marek; Gupta, Kapuganti Jagadis; Wendehenne, David; Loake, Gary J.; Durner, Jorg; Lindermayr, Christian; Molnár, Árpád; Kolbert, Zsuzsanna; Oliveira, Halley C.
Nitric oxide (NO) is a multifunctional gaseous signal that modulates the growth, development and stress tolerance of higher plants. NO donors have been used to boost plant endogenous NO levels and to activate NO-related responses, but this strategy is often hindered by the relative instability of donors. Alternatively, nanoscience offers a new, promising way to enhance NO delivery to plants, as NO-releasing nanomaterials (e.g., S-nitrosothiol-containing chitosan nanoparticles) have many beneficial physicochemical and biochemical properties compared to non-encapsulated NO donors. Nano NO donors are effective in increasing tissue NO levels and enhancing NO effects both in animal and human systems. The authors believe, and would like to emphasize, that new trends and technologies are essential for advancing plant NO research and nanotechnology may represent a breakthrough in traditional agriculture and environmental science. Herein, we aim to draw the attention of the scientific community to the potential of NO-releasing nanomaterials in both basic and applied plant research as alternatives to conventional NO donors, providing a brief overview of the current knowledge and identifying future research directions. We also express our opinion about the challenges for the application of nano NO donors, such as the environmental footprint and stakeholder’s acceptance of these materials.
Recommendations on terminology and experimental best practice associated with plant nitric oxide research
(John Wiley & Sons, 2020) Gupta, Kapuganti Jagadis; Hancock, John T.; Petrivalsky, Marek; Kolbert, Zsuzsanna; Lindermayr, Christian; Durner, Jorg; Barroso, Juan B.; Palma, José M.; Brouquisse, Renaud; Wendehenne, David; Corpas, Francisco J.; Loake, Gary J.
Nitric oxide (NO) emerged as a key signal molecule in plants. During the last two decades impressive progress has been made in plant NO research. This small, redox‐active molecule is now known to play an important role in plant immunity, stress responses, environmental interactions, plant growth and development. To more accurately and robustly establish the full spectrum of NO bioactivity in plants, it will be essential to apply methodological best practice. In addition, there are some instances of conflicting nomenclature within the field, which would benefit from standardisation. In this context, we attempt to provide some helpful guidance for best practice associated with NO research and also suggestions for the cognate terminology.
Regulating the regulator: nitric oxide control of post-translational modifications
(John Wiley & Sons, Inc., 2020) Gupta, Kapuganti Jagadis; Kolbert, Zsuzsanna; Durner, Jorg; Lindermayr, Christian; Corpas, Francisco J.; Brouquisse, Renaud; Barroso, Juan B.; Saima, Umbreen; Palma, José M; Hancock, John T.; Petrivalsky, Marek; Wendehenne, David; Loake, Gary J.
Nitric oxide (NO) is perfectly suited for duties as a redox signalling molecule. A key route for NO bioactivity occurs via protein S‐nitrosation, the addition of a NO moiety to a protein cysteine (Cys) thiol (‐SH) to form a S‐nitrosothiol (SNO). This process is thought to underpin a myriad of cellular processes in plants linked to development, environmental responses and immune function. Here we collate emerging evidence showing that NO bioactivity regulates a growing number of diverse post‐translational modifications (PTMs) including SUMOylation, phosphorylation, persulfidation and acetylation. We provide examples of how NO orchestrates these processes to mediate plant adaptation to a variety of cellular cues.