Browsing by Author "Haider, Toshiba"

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    Integrated seed proteome and phosphoproteome analyses reveal interplay of nutrient dynamics, carbon-nitrogen partitioning and oxidative signaling in chickpea
    (John Wiley & Sons, 2020) Sinha, Arunima; Haider, Toshiba; Narula, Kanika; Ghosh, Sudip; Chakraborty, Niranjan; Chakraborty, Subhra
    Nutrient dynamics in storage organs is a complex developmental process that requires coordinated interactions of environmental, biochemical, and genetic factors. Although sink organ developmental events have been identified, our understanding of translational and post‐translational regulation of reserve synthesis, accumulation and utilization in legume crops is limited. To understand nutrient dynamics during embryonic and cotyledonary photoheterotrophic transition to mature and germinating autotrophic seeds, an integrated proteomics and phosphoproteomics study in six sequential seed developmental stages in chickpea was performed. MS/MS analyses identified 109 unique nutrient‐associated proteins (NAPs) involved in metabolism, storage and biogenesis, and protein turnover. Differences and similarities in 60 nutrient‐associated phosphoproteins (NAPPs) containing 93 phosphosites were compared with NAPs. Data revealed accumulation of carbon‐nitrogen metabolic and photosynthetic proteoforms during seed filling. Furthermore, enrichment of storage proteoforms and protease inhibitors was associated with cell expansion and seed maturation. Finally, combined proteoforms network analysis identified three significant modules, centered around malate dehydrogenase, HSP70, triose phosphate isomerase and vicilin. Novel clues suggest that ubiquitin‐proteasome pathway regulates nutrient reallocation. Second, increased abundance of NAPs/NAPPs related to oxidative and serine/threonine signalling indicate direct interface between redox sensing and signaling during seed development. Taken together, nutrient signals act as metabolic and differentiation determinant governing storage organ reprogramming.
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    Seed proteomics: An overview
    (Springer, 2016) Narula, Kanika; Sinha, Arunima; Haider, Toshiba; Chakraborty, Niranjan; Chakraborty, Subhra
    Seed is vital for propagation of spermatophytes in biome and as food source for inhabitants of the earth. Studies on seed proteins provide platform for new avenues to explore molecular networks and pathways governing seed filling, maturation, germination, and seedling formation. Protein expression changes of three genetically different sub-regions of angiosperm seeds are reflected in ordered chain of biological events represented from family differences in different taxas. Different families of angiosperm show divergence of seed protein evolution and thus provide insights into seed structure and function. A gamut of information is available on seed proteomic datasets from approximately 3500 proteins that impinge on protein function in diverse plant families. The functional modularity of seed proteins were compared amongst species that span from dicot to moncot and diploid to polyploid. Transitions of protein complement revealed difference between dormancy and germination towards understanding biological check point at translational level. Goal of this chapter is to critically review data available till date on seed proteomic studies and identify family and cross genera knowledge gaps. The information thus obtained would unravel new components and an unparallel understanding of the molecular processes underlying translational and post-translational variations under different conditions that involves histodifferentiation and organogenesis of the seed.