Browsing by Author "Chaudhry, Vaishali"

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    Characterization of DEAD-box family of RNA helicases in tomato provides insights into their roles in biotic and abiotic stresses
    (Elsevier B.V., 2019) Pandey, Saurabh; Muthamilarasan, Mehanathan; Sharma, Namisha; Chaudhry, Vaishali; Dulani, Priya; Shweta, Shweta; Jha, Sarita; Mathur, Saloni; Prasad, Manoj
    In plants, RNA helicases play significant roles in growth, development and stress response. In a previous study, a three-fold upregulation of a DEAD-box RNA helicase in a tomato cultivar tolerant to Tomato leaf curl New Delhi virus (ToLCNDV) as compared to susceptible cultivar during virus infection was shown. Given this, a comprehensive study was performed to identify the members of RNA helicase family in tomato and analyze their functional properties in response to abiotic stresses, hormone treatments and ToLCNDV infection. A total of 131 genes were identified and classified into DEAD- (42), DEAH- (38), and DExD/H-box (51) RNA helicases. Expression profiling of candidate genes in response to abiotic stresses and ToLCNDV infection in contrasting tomato cultivars suggested the putative roles of SlDEAD23 and SlDEAD35 in biotic and abiotic stresses. Heterologous overexpression of these genes in yeast enhanced the tolerance of transgenic cells to salt and cold stresses. Further, virus-induced silencing of SlDEAD35 in ToLCNDV tolerant cultivar resulted in susceptibility to virus infection, thus suggesting its involvement in tolerance mechanism. Altogether, this study provides novel insights into the structure, organization and involvement of DEAD-box RNA helicase genes in biotic and abiotic stress responses in tomato.
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    Genomics resources for abiotic stress tolerance in solanaceae crops
    (Springer, 2017) Sharma, Shambhavi; Pandey, Saurabh; Muthamilarasan, Mehanathan; Chaudhry, Vaishali; Dulani, Priya; Prasad, Manoj
    The study of abiotic stresses in plants is crucial for an understanding of the mechanisms involved in responses to these stresses. The complex nature of abiotic stress-related traits and the occurrence of more than one stress simultaneously further complicate the study. The availability of genomic tools and resources allows a leap in plant breeding by facilitating the study of the genotype and its relationship with the phenotype. The development of techniques such as Next-Generation Sequencing (NGS) allowed the sequencing of genomes of cultivars and their wild relatives, enriching the available genetic as well as genomic resources. Genome-wide discovery of markers and quantitative trait loci are used for marker-assisted selection and breeding. The availability of the genome sequence information has expedited several downstream analyses, including genome-wide identification and expression profiling of the genes associated with stress response. This is coupled with the use of mutants and transgenics to elucidate and verify the function of genes in a high-throughput fashion. In this chapter, the progress made in the generation and enrichment of genomic resources of Solanum tuberosum and S. lycopersicum are discussed from the point of view of genetic improvement for abiotic stress tolerance.